Chapter 1 Spectrophotomerty 1.1 Basic Concepts and Principle of Spectrophotometry 1.2 Measurement of light Absorption 1.3 Aplications of Spectrophotomerty EXP.1 Quantitative Analysis of Protein EXP.2 Determination of Blood Glucose Chapter 2 Electrophoresis 2.1 Basic Principles of Electrophoresis 2.2 Impact Factors of Electrophoresis 2.3 Detection of Components after Electrophoreetic Separation 2.4 Special Electrophoreetic Techniques and Aplications EXP.3 Separation of Serum Proteins by Cellulose Acetate Membrane Electrophoresis EXP.4 Separation of Serum Lipoprotein by Agarose Gel Electrophoresis EXP.5 Proteins Seperation by SDS-Polyacrylamide Gel Electrophoresis Chapter 3 Chromatography 3.1 Fundamental Principles of Chromatography 3.2 Commonly Used Chromatographic Techniques EXP.6 Detecting Transamination of Amino Acids by Paper Chromatography EXP.7 Separation of Mixed Amino Acids by Cation Excharge Chromatography EXP.8 Separation of Hemoglobin and Dinitrophrnyl-Glutsmste by Gel Filtration Chromatography Chapter 4 Enzyme Analysis 4.1 The Activity of Enzyme 4.2 Enzyme Kinetics 4.3 Enzymatic analysis EXP.9 Assay the Activity of ALT in Serum EXP.10 LDH Analysis EXP.11 Effect of Substacte Concentration on Enzyme Activity-Determining Km Value for Alkaline Phosphatase EXP.12 Coupled enzymatatic raaction assay-Determination of Total Choleseterol,Triglycerides and Glucose in Serum Chapter 5 Isolation and Purification of Protein 5.1 Methods and Basic Principles of Protein Purification 5.2 Procefure of Protein Preparation 5.3 Crystallization,concentration,Drying,and Storage of Protein 5.4 Identification and Analysis of Protein EXP.13 Isolation and Identification of Serum IgG EXP.14 Expression, Purificationand Identification of Glutatathione S-Transferase Fusion Protein Chapter 6 Isolation, Purification and Identification of Nucleic Acids 6.1 Isolation, Purificationand of Nucleic Acids 6.2 Identification and analyusis of Nucleic Acids EXP.15 Isolation of Eukaryotic Genomic DNA by Proteinase K-Phenol or NAI Method EXP.16 SDS-Alkaline Lysis of Plasmid DNA EXP.17 Isolation of Total RNA by TRIzol Reagent EXP.18 Identification of DNA by UV-Sepctrophotomentry and Gle Electrophoresis EXP.19 Identification of RNA by UV-Sepctrophotomentry and Formaladehyde Denaturating Agerose Gle Electrophoresis EXP.20 Polymerase Chain Reaction and Reverse Transcription-Pcr Chapter 7 Genetic Engineering 7.1 The Process of Gene Cloning 7.2 Enzymes for Gene Cloning 7.3 Vectors for Gene Cloning 7.4 Expression of Foreign Genes and Purification of Recombinant Protweins EXP.21 DNA Cloning EXP.22 Expression of Exogenous Gene in E.coli and Purification of the Expressional Product English-Chinese Index
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